Application

This rat monoclonal Anti-mouse IFN gamma Antibody, APC, clone XMG1.2, Cat. No. MABF1515 is validated for use in Flow Cytometry, for the detection of IFN gamma.

General description

The XMG1.2 antibody is specific for mouse Interferon-gamma (IFN-g), a 20 kDa type II cytokine known for its central roles in protection against bacterial or viral pathogens and for its anti-tumor properties. IFN-g is secreted by several types of immune cells, which allow the cytokine to modulate innate immunity, when secreted by NK and NKT cells, and to function in support of adaptive immunity when secreted by Th1 and CD8+ T cells (CTLs).The XMG1.2 antibody is suitable for detection of intracellular IFN-g protein, e.g. by flow cytometry, as well as for quantitative analysis of the secreted protein by ELISA, when paired with an appropriate secondary antibody. This clone is also widely used for neutralization of the functional activity of IFN-g in a variety of assays.

Other Notes

Concentration: Please refer to lot specific datasheet.

Quality

This monoclonal antibody was purified from tissue culture supernatant via affinity chromatography. The purified antibody was conjugated under optimal conditions, with unreacted dye removed from the preparation.

Flow Cytometry Analysis: 0.06 µg from a representative lot detected IFN gamma in one million C57Bl/6 splenocytes.